Journal: BBA - Molecular Basis of Disease

Key Words: enhancer; pleiotropy; proliferation, migration, non-small cell lung cancer

Abstract: Enhancers, cis-acting DNA elements for transcriptional regulation, are important regulators of cell identity and disease. However, of the hundreds of thousands of enhancers annotated in the human genome, only a few have been studied for their regulatory mechanisms and functions in cancer progression and therapeutic resistance. Here, we report the pleiotropy of one enhancer (named enh9) in both cell proliferation and migration in non- small cell lung cancer (NSCLC) cells. By integrating multi-genomic data, ERMP1 and PD-L1 were screened out as potential targets of enh9. CUT&Tag sequencing demonstrated that enh9 was involved in the genomic interactions between the transcription factor RELA and the promoters of ERMP1 and PD-L1. In addition, ERMP1 and PD-L1 were validated to be involved in cell proliferation and migration, respectively. Our study fully elucidated the function and transcriptional regulation mechanisms of enh9 in NSCLC. The exploration on enhancers is promising to provide new insights for cancer diagnosis and therapy.

Co-author: Qian Liu,Yingying Duan,Sihan Qi

First Author: Qilin Wang,Junyou Zhang,Yanling Wen

Indexed by: Journal paper

Correspondence Author: Chunyan Li

Volume: 1870

Page Number: 167015

Translation or Not: no

Date of Publication: 2024-01-04

Included Journals: SCI